An important worry is ESKAPE pathogens, an acronym for a group of seven significant nosocomial pathogens, including Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp. which were declared by the World Health Organization as priority pathogens. Bacteriophages, or bacterial viruses, are employed in phage treatment, a promising alternative, to destroy bacteria. To combat Carbapenem Resistant Enterobacteriaceae (CRE), particularly Klebsiella sp., in this study, bacteriophages were isolated and characterised for their lytic activity against CRE. Using routine antibiotic susceptibility testing, 100 clinical isolates were examined for their resistance patterns. The isolates that are resistant to Meropenem and colistin, the last resort drugs for gram negative pathogens, were subsequently chosen for phage isolation. The source for the enrichment procedure utilised to isolate bacteriophages was a sample of sewage. Against clinical Klebsiella sp. isolates that were multidrug resistant, a total of seven phages were discovered. In terms of host range, stability, life cycle, and bacterial-phage growth dynamics, four phages were fully defined. To determine if bacteriophage therapy was efficient in eradicating biofilm, CRE isolates were also examined for their capacity to create biofilms, and phage treatment was provided. The isolated phages are stable in a range of temperatures (-20°C to 50°C) and pH (5–9) and have a limited host range. All four phages had short latency times (less than 40 minutes) and short adsorption times (less than 20 minutes), together with large burst sizes. Phage kinetics demonstrated that at higher MOIs, bacterial growth is hindered, and that as the MOI falls, bacterial OD reduction becomes less prominent. As a result of this study, the isolated phage can be used as a successful therapeutic candidate to treat Klebsiella sp., infections. We can say that when antibiotic treatment does not work, using phages to treat resistant infections will be a promising alternative.

Key words- drug resistance, antibiotics, bacteriophages, Klebseilla sp.,