In this work, zinc oxide (ZnO) nanoparticles were synthesized via green route using clerodendrum inerme and discorea alata leaves extract. The structure, functional group and absorption spectra were explored using X-ray diffractometer (XRD), Fourier Transformed Infrared Spectroscopy (FTIR) and UV-Vis spectroscopy. The XRD pattern confirmed the hexagonal wurtzite structure with an average crystallite size (D) of 24 nm and 29 nm and the functional group analysis (FTIR) revealed the metal oxides vibrations at 465 cm^-1 and 455 cm^-1 explored clerodendrum inerme (CI) and discoreaalata (DA) mediated CI-ZnO and DA-ZnO nanoparticles respectively. The UV-Vis analysis revealed the absorption peaks at 346 nmand 352 nm, and their band gap values are found to be 3.1 eV and 3.2 eV. XPS analysed the surface of the nanoparticles and revealed the elemental compositions, binding energy and oxidation states of ZnO nanoparticles. The bio-medical activities such as anti-inflammatory and anti-diabetic analysis of the synthesized ZnO nanoparticles were examined by Bovine albumin serum (BSA) denturation and α-amylase inhibition techniques. The highest inhibition percentage of 98.97 % and 99.90 % are obtained against α-amylase and bovine serum albumin respectively. Further, the MTT assay revealed the decreased cell viability of MCF-7 breast cancer cell lines at the higher concentrations. Hence, the green synthesized ZnO nanoparticles can serve as an anti-cancer, anti-diabetic and anti- inflammatory agents and can envisaged as a potential drug for anti-cancer and other biomedical applications.

Keywords: Anti-Cancer, Green synthesis, ZnO nanoparticles