Cimicifuga racemose (Black cohosh) expounds several pharmacodynamic impacts related to its medicinal and curative efficacy.  However, the bioactives associated with the preventive and beneficial properties of Cimicifuga racemose are not well correlated.  Hence, in this study, we conducted a meta-analysis and in-silico analysis to identify the potent bioactives present in Cimicifuga racemose related to its prophylactic and therapeutic effects.  Consequently, the major bioactives of Cimicifuga racemose are triterpene glycosides, acetein and 27-deoxyactein, and the isoflavone, formononetin.  The current analysis will reveal the gastrointestinal tract (GIT) and dermal route absorption profile of these potent bioactives and their interactions with P-glycoprotein and cytochrome-P450 system (CYP1A2, CYP2C19, CYP2C9, CYP2D6, and CYP3A4).  Thus, based on the previous studies and existing literature, Cimicifuga racemosa has been used to prevent and treat menopausal symptoms.  Thus, the bioactives of Cimicifuga racemose can selectively affect targets in the human physiological system leading to its potent prophylactic and therapeutic effects.