Calebin-A (hydroxycinnamic acid, novel curcuminoid) is a natural bioactive found in turmeric (Curcuma longa) belonging to the family Zingiberaceae. Calebin-A has been mainly shown to systematically interact with p65 and influence the NF-κB pathway. Due to the above pharmacological mechanism of action, Calebin-A has been therapeutically used in the treatment of cancer and tumor suppression. Yet, there are very few scientific reports on the pharmacodynamic effects of Calebin-A in the brain (central nervous system). To date, only a single report investigated the neuroprotective effect against β-amyloid-induced in vitro neurotoxicity. Thus, there is a significant gap in the neuropharmacological effect of Calebin-A. Hence, this study elucidated the neuro-pharmacodynamic actions of Calebin-A on the dopaminergic and hippocampal neurons. Furthermore, in-silico analyses were performed to validate its neuro-pharmacodynamic properties. Calebin-A was incubated with different doses at two different time points to establish the effect on dopaminergic and hippocampal neuronal viability. Endogenous neurotoxin, hydrogen peroxide was used as a positive control. Statistical analysis was performed using Prism-V software. The current in-silico and in vitro studies established the pharmacokinetic profile and the effect of Calebin-A on dopaminergic and hippocampal neuronal viability.