Pterostilbene (dimethyl ether analog of resveratrol)is metabolically stable and displayed substantially superior pharmacological activities as compared to the well-established natural bioactive, resveratrol. 3'-Hydroxypterostilbene (a natural pterostilbene analog and a metabolite) is a known apoptosis activatorand autophagy inducer.  3'-Hydroxypterostilbene inhibits Sirtuin, PI3K/Akt/mTOR/p70S6K, and p38MAPK pathways and activates the ERK1/2, JNK1/2 MAPK pathways.  The current literature reveals it to possess antimicrobial (anti-fungal), anti-obesity, and anti-cancer (skin, leukemia, and colon) activities. At present, there are no reports on the actions of 3'-Hydroxypterostilbene on the central nervous system. Consequently, there is a void in the neuropharmacological effect of 3'-Hydroxypterostilbene.  Therefore, this research study investigated the effect of 3'-Hydroxypterostilbene on the dopaminergic and hippocampal neurons.  Additionally, in-silico analyses were performed to validate its neuro-pharmacodynamic effects.  3'-Hydroxypterostilbene was incubated with different doses at two different time points to establish the effect on dopaminergic and hippocampal neuronal viability.  Endogenous neurotoxin, hydrogen peroxide was used as a positive control.  Statistical analysis was performed using Prism-V software.  The in-silico and in vitro studies established the pharmacokinetic profile and the effect of 3'-Hydroxypterostilbene on dopaminergic and hippocampal neuronal viability.