Hydroxyapatite (HAp) is an eminent material of choice in several biomedical applications such as orthopedics, dentistry etc., due to identical mineral arrangement of natural bone. Addition of nanocarbon, the development of structural and chemical properties of HAp. It also enhances the bioactivity of HAp to make it as suitable for bone engineering, drug and gene delivery applications. In this work, HAp was chemically synthesized by incorporating nanocarbon through DC glow discharge plasma through plasma immersion system with different plasma processing parameters such as voltage, gas flow rate and processing time. The structural, morphological, chemical state and biological studies for the as-prepared and plasma processed samples were carried out using X-Ray Diffraction (XRD), Field Emission Scanning Electron Microscope (FESEM), X-ray Photoelectron Spectroscopy (XPS), and Simulated Body Fluid (SBF) test, cytotoxicity analysis respectively. HR-TEM and FESEM images shows the hexagonal shape and the carbon present in the core shell of HAp. In SBF test, FESEM results confirming the formation of smooth apatite particles over the nano-carbon HAp surface.