Scutellaria lateriflora is a native plant of North America and contains large amount of chemically active flavonoids than other commonly used species of the plant of genus Scutellaria. To date very limited research studies have been done to measure the immunopharmacological activities of S. lateriflora. The purpose of this study was to determine the immunomodulatory and anti-inflammatory properties of S. lateriflora. Experiments were designed to measure the effects of an alcoholic extract of S. lateriflora (SLE) on pro-inflammatory enzyme (cyclooxygenase-1-COX-1, cyclooxygenase-2-COX-2) and 15 Lipoxygenase-15-LOX Lipoxygenases) activities, activation of T lymphocytes and major histocompatibility complex class-II (MHC-II) expression in bovine monocyte-derived dendritic cells (MoDCs). The SLE inhibited COX-1, COX-2 and 15 LOX activities but did not affect T cell proliferation, MHC-II expression on MoDCs and pro-inflammatory cytokines production by T cells. Our results established the anti-inflammatory mechanisms of SLE, which validates the potential possibility of treatment for various inflammation driven diseases.