Proceedings of Technological Advances in Science, Medicine and Engineering Conference 2021

Designing a novel electrochemical impedance biosensors for the detection of infectious pancreatic necrosis virus
Saim Imran, Kagan Kerman
Abstract

Infectious pancreatic necrosis virus (IPNV) is a major disease-causing pathogen for farmed salmon worldwide. Due to its high infectivity and mortality, an accurate and sensitive detection for the virus can help isolate infected individuals. Herein, multiple designs for biosensors were reported as a point of care diagnostic for IPNV. VP2, an antigenic outer coat protein on IPNV, and anti-VP2 antibody were used as the immobilizing materials for the sensor. Gold screen printed electrodes (AuSPE) were functionalized with a self-assembled monolayer and followed by immobilization of either VP2 or anti-VP2 antibody, and then addition of anti-VP2 antibody or VP2 respectively. Electrochemical impedance spectroscopy (EIS) was used to determine successful binding, with the electrodes presenting promising results in both conditions. The procedure for antibody immobilization was modified using protein A as a step prior to antibody immobilization, as this allowed for proper orientation of the antibody, improving antigen recognition as a result. Finally, molecularly imprinted polymers were fabricated on AuSPE via electropolymerization of aniline in presence of VP2 antigen, to provide a more stable solution to the biosensor design. Sites created in the polymer should allow VP2 to bind, and this was confirmed via EIS studies. Stability results for the study indicate polymer stability over two months. These methods provide encouraging results for the detection of IPNV without pre-treatment and as a point of care diagnostic. Further studies to determine the limits of detection and analysis on real sample should be performed to test viability of these methods.

 


Last modified: 2021-06-27
Building: TASME Center
Room: Science Hall
Date: July 3, 2021 - 04:35 PM – 04:50 PM

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